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BP: Fachverband Biologische Physik
BP 27: Biotechnology and Bioengineering
BP 27.7: Vortrag
Donnerstag, 14. März 2013, 11:30–11:45, H43
Tailoring substrates for long-term organotypic culture of adult neuronal tissue — •Mareike Zink1, Valentina Dallacasagrande1,2, Alexander Jakob3,4, Marcus Müller3, Andreas Reichenbach2, Josef Käs1, and Stefan G. Mayr3,4 — 1Institut für Experimentelle Physik 1, Universität Leipzig — 2Paul-Flechsig-Institut, Universität Leipzig — 3Leibniz Institut für Oberflächenmodifizierung e.V., Leipzig — 4Translationszentrum für Regenerative Medizin, Fakultät für Physik und Geowissenschaften, Universität Leipzig, Leipzig, Germany
Organotypic tissue cultures establish a highly promising approach for performing in vivo type of studies in vitro. However, very limited survival times of only a few days for adult tissue often limit their application. We propose a novel biotechnological concept, which allows for unprecedented long culture times even in absence of biochemical growth factors. Employing TiO2 nanotube array substrates, whose interaction properties with individual cells and the overall tissue can be tuned by the tube parameters, we verify this concept for different adult neuronal explants, which are successfully cultured for the first time longer than 14 days with no indications of degeneration. It turned out that adequate nanotube diameter, wall thickness and surface roughness vary for successful long-term culture of adult mammalian retinae and brain slices. Additionally, we found that the intrinsic super-hydrophilicity of the substrates allows for a continuous supply of fresh medium without perfusion systems. Our findings pave the way for in vitro drug testing, as well as neuronal tissue regeneration.