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BP: Fachverband Biologische Physik

BP 27: Biotechnology and Bioengineering

BP 27.8: Vortrag

Donnerstag, 14. März 2013, 11:45–12:00, H43

Advanced high-throughput SEIRA methodology for multiplexed bioassay assessment — •Andrea Hornemann¹, Sabine Flemig², Gerhard Ulm¹, and Burkhard Beckhoff¹ — ¹Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 10587 Berlin, Germany — ²BAM Bundesanstalt für Materialforschung und -prüfung, Richard-Willstätter-Str.10, 12489 Berlin, Germany

Nanotechnology has an increasing impact in both the design and functionality of biomedical and biodiagnostic devices for a reliable high-performance analysis. High-throughput technological advances in diagnostic assays of high complexity induce requirements on selectivity, sensitivity, and multiplexing capability that will be the main challenges for the prospective assay design. We discuss the capability of surface-enhanced infrared absorption (SEIRA) spectroscopy as versatile diagnostic readout tool for both its potential widespread point-of-care use and application for the multiplexed bioassay assessment. We performed a qualification study on NP-enhanced SEIRA, enabling the readout of tunable assays. We utilized synchrotron radiation as its high brilliance provides an improved quality of spectral datasets and low data acquisition times. Our analysis included a profound study on nanoscaled biolabels that exhibit distinct narrow emission profiles of the incorporated antibody-fluorophore complexes. The molecular fingerprints have been successfully analyzed by multivariate methods with respect to their multiplexing capabilities. This robust spectral encoding by SEIRA signatures is expected to open new opportunities for a fast, reliable and multiplexed high-end screening in biodiagnostics.