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BP: Fachverband Biologische Physik

BP 28: Focus session: Intracellular Spectroscopy

BP 28.7: Topical Talk

Donnerstag, 14. März 2013, 11:30–12:00, H44

Looking at proteins inside live cells with atomic resolution: Science fiction or science reality? — •Phil Selenko — FMP Berlin

A protein's structure and its dynamic behavior are inherently dependent on its physiological environment. While most proteins function inside live cells, our knowledge about them is largely based on experiments that bear little resemblance to the physical and biological properties of a cell. A new approach in liquid-state NMR spectroscopy, in-cell NMR, now offers convenient means to directly study proteins at atomic resolution inside live cells. In this lecture, I will introduce the basic concepts behind in-cell NMR spectroscopy and provide examples of the structural and dynamic characteristics of a human amyloid protein in five different mammalian cell types, including dopaminergic neurons.