Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 30: Tissue
BP 30.6: Vortrag
Donnerstag, 14. März 2013, 16:45–17:00, H43
Morphogenesis and ageing of MDCK epithelial tissues depend on substrate elasticity — •Sara Kaliman1, Christina Jayachandran2, Damir Vurnek1, Florian Rehfeldt2, and Ana-Sunčana Smith1 — 1Institute for Theoretical Physics, University Erlangen-Nürnberg, Germany — 23rd Institute of Physics-Biophysics, University ofGöttingen, Germany
Morphogenesis of epithelial tissues is the key to understanding tissue development and regeneration, or tumour growth. It is believed to be dominated by intercellular interactions, and hence, independent of substrate rigidity. However, here we show that different regimes of growth occure on soft and hard substrates. Substrates with a rigidity higher than 5 kPa promote radially growing clusters, which in early stages expand exponentially with a persistantly low density of cells. When the cluster radius exceeds 5 mm, its area increases linearly in time. During that period, a bulk tissue of higher density forms in the center of the cluster, whereas the edge remains at a constant low density, independently of the cluster size. On 1 kPa substrates the cells initially form small multilayered dropplets that, if sufficiently large, nucleate a very dense and well structured monolayer in its center. These clusters expand to macroscopic sizes by adopting irregular shapes, while mantaining the initial monolayer morphology. In both cases, tissues age, the signature of which are (i) an inhomogeneous density, and (ii) nuclei that deform strongly due to the substrate sensitive restructuring of the actin cortex. Furthermore, dome-like and tubule-like structures are found on hard substrates, while soft substrates promote anoikis.