Regensburg 2013 – scientific programme
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BP: Fachverband Biologische Physik
BP 8: Posters: Proteins
BP 8.3: Poster
Monday, March 11, 2013, 17:30–19:30, Poster B2
Time-Resolved FTIR Difference Spectroscopy of Vibrational Control Experiments on Bacteriorhodopsin — •Christian Bauer1,2, Michael Gensch2, and Joachim Heberle1 — 1Freie Universität Berlin — 2HZDR
We aim at investigating how photoreactions of proteins can be controlled by means of intense THz radiation tuned in resonance to specific vibrational modes, much in analogy to coherent control experiments conducted by fs NIR laser pulses [1]. Bacteriorhodopsin is the sole protein of the purple membrane of the archaebacterium Halobacterium salinarum [2]. Upon illumination, its chromophore retinal isomerizes around the C13-C14 double bond [3] and the protein undergoes a sequence of intermediate states which is called a photocycle. For investigation of this photocycle we combined a time-resolved IR difference spectroscopic setup using the step-scan technique [4] with intense, tunable narrow bandwidth THz radiation at the ps beamline of the THz free electron laser FELBE [5]. In our experiments, the photoreaction is initiated by a visible laser pulse as in standard experiments, but then the sample will be irradiated by a THz pulse from the free electron laser tuned into resonance with low-energy vibrational modes which is supposed to influence the photoreaction [1].
[1] Prokhorenko V.I. et al. Science 313, 1257 (2006) [2] Oesterhelt D. et al. PNAS 70, 2853 (1973) [3] Stoeckenius W. et al. Biophys. Struct. Mech. 3, 65 (1977) [4] Radu I. et al. Photochem. Photobiol. Sci. 8, 1517 (2009) [5] Bauer, C. et al. Journal of Physics Conference Series, 359, 012011 (2012)