Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 9: Posters: Membranes
BP 9.5: Poster
Montag, 11. März 2013, 17:30–19:30, Poster B2
Single lipid molecule tracking on suspended lipid membranes — •Jens Ehrig1, Susann Spindler1, Cornelia Becker1, Chia-Lung Hsieh2, and Vahid Sandoghdar1 — 1MPI for the science of light, Erlangen, Germany — 2Institute of Atomic and Molecular Sciences, Academia Sinica, Taiwan
We study the diffusion of single lipid molecules in free-standing lipid membranes by means of interferometric scattering microscopy (iSCAT)[1]. Free-standing aperture-spanning membranes are obtained by bursting giant unilamellar vesicles on a holey Si3N4 support[2]. The diffusion of lipids is studied by recording the scattering signal from gold nanoparticles (AuNP) bound to various molecules in the membrane including the ganglioside GM1 as well as phospholipids with a biotinylated headgroup. The AuNP are bound to GM1 via cholera toxin subunit B (CTxB) or antibodies. In case of the biotinylated phospholipids the biotin directly binds to the streptavidin-covered AuNP. Using the iSCAT technique we are able to resolve the motion of single lipid molecules at a high spatial and temporal resolution of below ∼2 nm and ∼1 µs, respectively. The results from measurements on free-standing membranes are compared to the ones obtained on supported lipid bilayers.
[1] K. Lindfors et al. Phys Rev Lett 93 2004; P. Kukura et al. Nat Methods 6 2009; [2] F. Heinemann and P. Schwille ChemPhysChem 12 2011