Regensburg 2013 – scientific programme
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 25: Biomaterials and Biopolymers II (joint session BP/CPP)
CPP 25.3: Talk
Wednesday, March 13, 2013, 10:15–10:30, H43
Influence of direct laser written three-dimensional topographies on osteoblast-like cells — •Judith K. Hohmann1, Erik H. Waller1, Rainer Wittig2, Rudolf Steiner2, and Georg von Freymann1 — 1Physics Department and Research Center OPTIMAS, University of Kaiserslautern — 2Institute for Laser Technologies in Medicine and Metrology (ILM) at the University of Ulm
Biological cells react to various signals of their environment. While biochemical pathways have been investigated for decades, the influence of physical characteristics of the cellular environment has only been studied in the very recent past [1]. Especially information on the interaction with three-dimensional structures is barely available, since common chemical and/or physical surface treatments (e.g. acid-etching, sand blasting) lead to randomly shaped surface topographies. In general, results generated in such two-dimensional systems can hardly be transferred to natural, three-dimensional conditions.
Our well-defined three-dimensional templates are fabricated by direct laser writing and coated with titanium dioxide via atomic layer deposition. This allows us to provide biocompatible substrates.
We aim at understanding the relation between various three-dimensional structures and viability parameters of osteoblastic cells. To observe cellular behavior, SaOs-2 osteosarcoma cells are seeded onto the structures in order to test proliferation, morphology, adhesion and differentiation via fluorescence and staining techniques. These results might lead to novel dental implant surfaces which promote osseointegration. [1]Nikkhah et al. Biomaterials 33 (2012) 5230-5246