Regensburg 2013 – scientific programme
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 34: Poster: Wetting, Micro and Nano Fluidics
CPP 34.16: Poster
Wednesday, March 13, 2013, 16:30–18:30, Poster C
Acoustic Sorting of Drops and Cells — •Lothar Schmid1 and Thomas Franke1,2 — 1Universität Augsburg, Experimentalphysik I, Soft Matter Group, Augsburg, Germany — 2Department of Physics and School of Engineering and Applied Science, Harvard University, Cambridge, USA
We use a surface acoustic wave (SAW) device for manipulating fluids, droplets and cells in microfluidic channels.
The acoustic wave is coupled into the channel at specific regions, generating pressure gradients or microvortices. Electric control of the wave generator allows for precise regulation and fast switching times.
We show an application of the technique by demonstrating a microfluidic fluorescence-activated sorter for cells and droplets at high rates. Our acoustic sorter combines the advantages of traditional fluorescence-activated cell sorting (FACS) and droplet sorting (FADS) and is applicable to a multitude of objects. We can sort aqueous droplets at rates as high as several kHz into two or more outlet channels. We demonstrate cell sorting in one-phase flow using fibroblasts cells without prior encapsulation into drops directly from the bulk phosphate buffered saline media. Our acoustic micro-FACS meets conventional laboratory practice but at the same time allows for modular integration into more complex microfluidic setups.