Regensburg 2013 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

CPP: Fachverband Chemische Physik und Polymerphysik

CPP 40: Focus: Van der Waals at soft matter interfaces: structure and dynamics

CPP 40.3: Hauptvortrag

Donnerstag, 14. März 2013, 10:15–10:45, H39

The influence of van der Waals interactions on the adsorption of proteins to solid/liquid interfaces — •Hendrik Hähl — Institute of Physical Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland

Protein adsorption to interfaces is an ubiquitous phenomenon. In particular on solid/liquid boundaries, adsorbed proteins precondition the surface for a later biofilm colonization. Therefore, a better understanding of this first step in biofilm development is highly desirable. The adsorption of proteins to the solid/liquid interface is driven by the interactions between proteins and substrates. Yet, particularly van der Waals (vdW) forces and their dependence on the substrate’s composition are mostly neglected in experiments as well as in simulations.

For the investigation of the influence of vdW forces on protein adsorption, we judiciously chose substrates, with which only the vdW potential could be varied. Therefore, silicon wafers with different silicon oxide thicknesses proved to be an excellent substrate system [1]. Adsorption kinetics as well as the structure of the final adsorbate of several globular proteins were determined on these substrates. Varying the oxide layer thickness caused changes in adsorption rates, course of the adsorption kinetics as well as in the density of the adsorbate irrespective of the surface energy [2]. Thus, it could be shown that the vdW forces between substrate and protein influence the adsorption process and the relaxation of the adsorbate in particular.
P. Loskill et al. Adv. Coll. Interf. Sci. 107 (2012) 179182.
H. Hähl et al. Langmuir 28 (2012) 7747.