Regensburg 2013 – wissenschaftliches Programm

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DY: Fachverband Dynamik und Statistische Physik

DY 36: Statistical Physics in Biological Systems IV (joint with BP)

DY 36.9: Vortrag

Freitag, 15. März 2013, 11:45–12:00, H44

Deducing underlying mechanisms from protein recruitment data — •Laurin Lengert and Barbara Drossel — TU Darmstadt, Hessen

The technique of fluorescently labelling proteins made it possible to visualize cellular proteins and to measure their distribution and dynamics within the cell. We focus on protein recruitment to a region in the cell following a triggering event, such as irradiation. Often mechanistic models are used to fit the recruitment data. In such models, differential equations describe the changes in the concentrations of activated or bound proteins in the region of interest. The aim of such mechanistic models consists in evaluating rate constants, in identifying the proteins and reactions that are essential for the investigated process, and in obtaining evidence for processes that are not directly visible. By analyzing in a systematic way the recruitment curves generated by different simple models, we explain how the features of the recruitment curves reflect the properties of the underlying processes. This analysis also shows that a distinction between different models is not always possible from a given set of data. However, in many cases it is possible to suggest additional experiments with different protein concentrations that allow to distinguish between different models.