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MA: Fachverband Magnetismus
MA 7: Magnetic Particles in Biomedical Diagnostics and Therapy (jointly with BP,CPP,ST)
MA 7.1: Hauptvortrag
Montag, 11. März 2013, 09:30–10:00, H22
Time-of-Flight Magnetic Flow Cytometry — •Michael Helou1,4, Mathias Reisbeck1, Lukas Richter1, Jacobus Bosch2, Roland Stauber3, Eckhard Quandt4, and Oliver Hayden1 — 1Siemens AG, Corporate Technology, 91058 Erlangen, Germany — 2Department of Medicine 5, Hematology and Medical Oncology, University Hospital Erlangen, 91054 Erlangen, Germany — 3Molecular and Cellular Oncology/Mainz Screening Center (MSC), Medical University Mainz, 55101 Mainz, Germany — 4Institute for Materials Science-Inorganic Functional Materials, Christian-Albrechts-Universität zu Kiel, 24143 Kiel, Germany
Flow cytometry - the gold standard for clinical single cell analysis - requires a point-of-care solution for decentralized applications. Here, we show a novel approach towards a time-of-flight magnetic flow cytometry using superparamagnetic iron-oxide nanoparticles (SPION) labeled cells. Within a microfluidic channel we are able to perform specific cell enrichment and cell focusing by magnetophoresis as well as single cell detection with integrated GMR sensors in a single step. We even take advantage of the magnetophoresis for an in-situ elimination of the background from non-bound markers. In addition, time-of-flight measurements of the labeled cells allow us to derive cell diameter information. Proof-of-concept of our magnetic flow cytometer is demonstrated with SPION labeled tumor cells spiked in stabilized whole blood.