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MA: Fachverband Magnetismus

MA 7: Magnetic Particles in Biomedical Diagnostics and Therapy (jointly with BP,CPP,ST)

MA 7.4: Vortrag

Montag, 11. März 2013, 10:45–11:00, H22

Direct Protein Detection in the Sample Solution by Monitoring Rotational Dynamics of Nickel Nanorods — •Stefan Schrittwieser¹, Frank Ludwig², Jan Dieckhoff², Andreas Tschoepe³, Annegret Guenther³, Michael Richter¹, Andreas Huetten⁴, Hubert Brueckl¹, and Joerg Schotter¹ — ¹AIT Austrian Institute of Technology, Vienna, Austria — ²TU Braunschweig, Braunschweig, Germany — ³Universitaet des Saarlandes, Saarbruecken, Germany — ⁴Bielefeld University, Bielefeld, Germany

We present experiments that demonstrate the feasibility of a recently introduced homogeneous immunodiagnostic approach to directly detect analyte binding by optical observation of the hydrodynamic properties of magnetically rotated nanorods ("PlasMag"). Specifically, we show that the phase lag of the long axis of nickel nanorods (magnetic core parameters: length 182 nm, diameter 26 nm) with respect to externally applied rotating magnetic fields significantly increases on the adhesion of bovine serum albumin (BSA) protein to their surfaces. To validate these results, we independently determine the amount of bound protein molecules by analysis of the electrophoretic mobility of the nanorods, which gives a protein surface density of 5.8 femtomol / mm².

Acknowledgements: The research leading to these results has received funding from the European Community’s 7th Framework Programme under grant agreement no 246479 (NAMDIATREAM).