Regensburg 2013 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 22: Nanostructures at Surfaces II
O 22.2: Vortrag
Montag, 11. März 2013, 16:15–16:30, H45
Multipotent mesenchymal stem cells (MSCs) and osteoblasts (OB) on SiO2 nanopillar arrays: Effect of pillar geometry on cell adhesion, proliferation and viability — •Burcin Özdemir1, Alfred Plettl1, Jörg Fiedler2, Jochen Bartholomä2, Paul Ziemann1, and Rolf Brenner2 — 1Institute of Solid State Physics — 2Departments of Orthopedics, University of Ulm, Germany
In this contribution, we quantitatively investigate the behavior of MSCs and OB as influenced by systematically nanostructured SiO2 surfaces in order to develop optimized interfacial interactions between cells and substrate for orthopedic device design. Diblock-copolymer micelle nanolithography (BCML) is applied in combination with photochemical growth to obtain highly ordered Au nanoparticles in 2D-arrays, which, subsequently, are used as nanomasks for anisotropic reactive ion etching (RIE). Cyclic combination of photochemical growth and RIE results in well-defined hexagonally ordered SiO2 nanopillar arrays with heights up to 320 nm (maximum aspect ratio ~9:1). Ten different SiO2 nanopillar arrays combining heights of 20 and 50 nm with interpillar distances of 50, 100, 120 nm and diameters of 10, 30 nm were analyzed by high resolution scanning microscopy and immunofluorescence. Highest proliferation rate for MSC was observed on the nanopillar arrays with dimensions of 10-100-50 nm (width, distance, height). Osteogenic differentiation, however, is significantly induced by shorter pillar heights.SEM examination showed that regardless of the 3D structure of the cells, they mainly adhered to the very tops of the pillars.