Regensburg 2013 – scientific programme
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O: Fachverband Oberflächenphysik
O 36: Poster Session II (Organic films and electronics, photoorganics; Nanostructures; Plasmonics and nanooptics, Surface chemical reactions and heterogeneous catalysis, Surface dynamics )
O 36.58: Poster
Tuesday, March 12, 2013, 18:15–21:45, Poster B2
New approach to the fabrication of ssDNA/oligo(ethylene glycol) monolayers and complex nanostructures — •M Nuruzzaman Khan1, Vinalia Tjong2, Ashutosh Chilkoti2, and Michael Zharnikov1 — 1Angewandte Physikalische Chemie, Universität Heidelberg, 69120 Heidelberg, Germany — 2Department of Biomedical Engineering, Duke University, N C 27708-0300, USA
Immobilization of single stranded DNA (ssDNA) on solid support is an important issue for binding and detection of the complementary DNA target as well as for the recognition of DNA binding proteins. Here we present a universal two-step approach to fabricate mixed monomolecular films comprising thiolated ssDNA and oligo(ethylene glycole) substituted alkanethiolates (OEG-AT) which exhibit biocompatible properties providing a suitable matrix for the ssDNA . At first, the primary OEG-AT monolayer was irradiated with electrons to create defects in a controlled fashion. In the second step, a defect-promoted exchange between the molecules in the monolayer and ssDNA species in solution occurred, with the extent depending on the irradiation dose. The approach was combined with E-beam lithography, which allowed us to fabricate versatile ssDNA patterns of any required shape imbedded in the protein-repelling matrix. Further, applying surface initiated enzymatic polymerization, we succeeded to amplify the above ssDNA/OEG-AT patterns in the z-direction in a controlled fashion. Both ssDNA pattern and related 3D nanostructures can be widely used as versatile nanofabrication platform in such important fields as bio-engineering, bio-technology, and sensor fabrication.