Berlin 2014 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 24: Biomolecules 2

MO 24.5: Vortrag

Freitag, 21. März 2014, 15:00–15:15, BEBEL SR144

Gas-Phase Spectroscopy of Conformer-Selected Proteins — •Stephan Warnke, Kevin Pagel, and Gert von Helden — Fritz-Haber-Institut der Max-Planck-Gesellschaft, Berlin, Germany

Mass spectrometry (MS) is a key technique to investigate biomolecules in the gas phase and many methods are available to obtain mass/charge ratios with very high accuracy. In combination with gas-phase spectroscopy, information about the molecules’ composition and local structure can be obtained. However, the overall 3-dimensional structure of the molecule cannot be investigated with these methods alone - even though their knowledge is crucial for the understanding of intra- and inter- molecular interactions as well as the function of the molecule in the living organism. Additionally, peptides and proteins can coexist in a multitude of different conformations in the gas phase and is is not clear in how far this structural heterogeneity does affect the above mentioned methods. A technique that is sensitive to the higher order structure of gas-phase ions is ion mobility spectrometry (IMS). In IMS the absolute (angle averaged) collision cross section and, thus, the effective size of the ion is determined. When molecules coexist in different conformations, they can be separated in space and time to allow for experiments on both mass/charge as well as shape/charge selected biomolecular ions.

In this talk, first results of spectroscopic experiments on mass/charge and conformer selected gas-phase proteins will be presented.