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MS: Fachverband Massenspektrometrie

MS 5: Molecules, Clusters, Decay and Reactions

MS 5.1: Hauptvortrag

Dienstag, 18. März 2014, 16:30–17:00, DO24 1.205

Quantitative Elemental and Molecular Mass Spectrometry for Biological Application — Sebastian Beck, Gunnar Schwarz, Renee Becker, David Benda, Violette Frochaux, Kathleen Schwarz, Katrin Brückner, and •Michael Linscheid — Humboldt-Universität zu Berlin, Dept. of Chemistry

When chromatographic techniques such as high pressure chromatography (HPLC) and electrophoresis were coupled directly to ICP-MS, elemental MS began to attract attention in biological research, since particularly heavy metals play an important role in biology and ICPS MS is one of the most precise methods in quantitative mass spectrometry. Thus, the analysis of metals and synthetic metal tags came into focus.Recently, we introduced metal tags carrying lanthanoides ("MeCAT") to quantify biopolymers using ICP-MS. Then, we investigated the fragmentation of MeCAT labeled peptide molecular ions and found characteristic fragments, which are formed upon infrared multiphoton dissociation. Hence, it should be possible to screen for MeCAT peptides in mixtures and distinguish between the different metals without the need for an element specific mass spectrometer. Most recently we extended the application range of the tagging technique to DNA oligomers allowing the detection and quantification of specific DNA sequences at very low levels. In this presentation the design of the techniques and the required instrumentation will be discussed. Then, strategies for application in biological mass spectrometry will be presented.Finally, the scope and limitations of this approach will be discusses in the light of most recent developments.