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BP: Fachverband Biologische Physik

BP 10: Posters: Molecular Motors

BP 10.3: Poster

Dienstag, 1. April 2014, 09:30–12:30, P1

Single and multi motor measurements of the kinesin-like protein KIF20A — •Alice Wiesbaum¹, Nikta Fakhri¹, I-Mei Yu², Anne Houdusse-Juille², and Christoph F. Schmidt¹ — ¹Georg-August Universität Göttingen, De — ²Institut Curie, Paris, Fr

The Kinesin-like protein KIF20A is necessary for different processes in human cells. It was found to have an essential role during cell division. There KIF20A is required for chromosome passenger complex mediated cytokinesis. Following phosphorylation by PLK1, KIF20A creates a docking site for PLK1 and recruits it at the central spindle. Knocking out KIF20A leads to defects in the cleavage furrow formation. A down-regulation of KIF20A showed an attenuate growth of pancreatic cancer cells. All this makes KIF20A an interesting object to study.

To understand the genetic structure and the function of single parts of KIF20A, we did in vitro experiments with different KIF20A mutants. KIF20A shows a flexible extension at the N-terminal, of which we partly removed different amounts of amino acids. We investigated KIF20A and the obtained constructs in surface gliding assays, as well as in single molecule tracking, using fluorescent nanotubes bound to the motor protein. In addition we had a look at the shape of KIF20A using small angle x-ray scattering (SAXS).