Dresden 2014 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 11: Posters: Cytoskeleton

BP 11.12: Poster

Dienstag, 1. April 2014, 09:30–12:30, P1

Subunit exchange in vimentin intermediate filaments — •Bernd Nöding and Sarah Köster — Institute for X-Ray Physics, University of Göttingen, Germany

Intermediate filaments, together with actin filaments and microtubuli and in combination with motorproteins and binding proteins make up the cytoskeleton of eukaryotic cells. While assembly mechanisms for different intermediate filaments have been modeled in the past, insights into the dynamic changes in the filament cross-section are still largely missing. Thus, we perform measurements of the exchange of fluorescently labeled subunits on fully assembled vimentin intermediate filaments. We find that an exchange of subunits occurs at a temperature dependent rate. Likely, polymorphism of the filament cross-section is an important factor in this process. With these findings, we aim to contribute to a more comprehensive description of the assembly and subunit exchange mechanism.