Dresden 2014 – scientific programme
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BP: Fachverband Biologische Physik
BP 11: Posters: Cytoskeleton
BP 11.13: Poster
Tuesday, April 1, 2014, 09:30–12:30, P1
Studying the assembly of intermediate filaments in microfluidic channels using fluorescence cross correlation spectroscopy (FCCS) — •Viktor Schroeder1, Bernd Nöding1, Anja Niedermayr2, Harald Herrmann3 und Sarah Köster1 — 1Institute for X-Ray Physics, Georg-August-University Göttingen, Göttingen, Germany — 2Department of Neurophysiology and Cellular Biophysics, Georg-August-University Göttingen, Göttingen, Germany — 3Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany
The cytoskeleton of eukaryotes consists of three different filamentous systems: microtubules, actin filaments and intermediate filaments (IFs). While both tubulin and actin are highly conserved, IF proteins occur in many different variations, depending on cell type and organism. All cytoskeletal filaments consist of distinct subunits and assemble in a characteristic way. For vimentin IFs, which are found in cells of mysenchymal origin, a principal assembly model exists. However, measurements of the assembly process with high time resolution, which would yield insight especially into the early assembly steps, are still largely missing. To approach this problem, we use fluorescence cross correlation spectroscopy (FCCS) in combination with microfluidic continuous flow mixers to access time scales in the millisecond range and directly follow binding events.