Dresden 2014 – scientific programme

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BP: Fachverband Biologische Physik

BP 12: Posters: Imaging

BP 12.5: Poster

Tuesday, April 1, 2014, 09:30–12:30, P1

Sub-diffraction imaging of cellular organelles — •Andreas Veres and Matthias Weiss — Experimental Physics I, University of Bayreuth, Bayreuth, Germany

Eukaryotic cells are highly compartmentalized. One of the most prominent organelles is the endoplasmic reticulum (ER) which assumes the morphology of a sponge-like web that spans throughout the cell. The full structure of the ER, however cannot be resolved by conventional light microscopy approaches as the typical mesh size is below the diffraction limit. To overcome this limitation, we have used SOFI (Superresolution optical fluctuation imaging) which provides a means to overcome the diffraction limit by exploiting fluorescence fluctuations of quantum dots. Based on our SOFI images we have determined the fractal dimensions of the ER and its porosity in cells at different stages.