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BP: Fachverband Biologische Physik
BP 12: Posters: Imaging
BP 12.6: Poster
Dienstag, 1. April 2014, 09:30–12:30, P1
Preparation of colloidal CdSe quantum dots for medical fluorescence labelling — •Svenja Herbertz1, Lothar Houben2, Kathrin Scheckenbach3, and Thomas Heinzel1 — 1Solid State Physics Laboratory, Buildg. 25.23., Heinrich-Heine University Düsseldorf, Universitätsstr. 1, D-40225 Düsseldorf — 2PGI-5, FZ Jülich, Wilhelm-Johnen-Str. , D-52425 Jülich — 3HNO-Klinik, Buildg. 13.76, Heinrich-Heine University Düsseldorf, Universitätsstr. 1, D-40225 Düsseldorf
The size dependence of their energy bandgap allows producing quantum dots of various fluorescence wavelengths in one production process. We fabricated colloidal CdSe QDs with tunable emission via kinetic growth with TOP as stabilizer [1] and investigated the influence of various growth parameters on the fluorescence behavior. The produced QDs have a size-dependent fluorescence line with typical FWHM of about 40 nm. Via X-ray diffraction and HR-TEM nanocrystal diameters of about 2 to 6 nm were measured and a hexagonal wurtzite crystal structure was identified. The surface of the QDs as-fabricated was chemically modified to establish solubility in aqueous media. In the context of detecting drugs in cell cultures by fluorescence labelling with QDs, an agent of particular interest is the chemotherapeutic cisplatin. The interaction of cisplatin with QDs, for a start without a direct connection of the molecules, was investigated. It was found that the presence of cisplatin quenches the fluorescence intensity of QDs in water.
[1] E. M. Boatman et al., J. Chem. Educ. 82, 1697(2005).