Dresden 2014 – scientific programme
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BP: Fachverband Biologische Physik
BP 12: Posters: Imaging
BP 12.7: Poster
Tuesday, April 1, 2014, 09:30–12:30, P1
Age related changes in human RPE cells, imaged by multicolor SIM microscopy. — •Florian Schock1,2, Gerrit Best1,2, Nil Celik2, Alina Bakulina6, Martin Hagmann1,2, Rainer Heintzmann4,5, Jürgen Hesser6, Stefan Dithmar2, and Christoph Cremer1,3 — 1Kirchhoff Institute for Physics, University of Heidelberg — 2Department of Ophthalmology, University of Heidelberg — 3Institute of Molecular Biology, Mainz — 4Institute for Physical Chemistry, University of Jena — 5Institute of Photonic Technology, Jena — 6Institute for Medical Technology, Mannheim
In our society old-age diseases are becoming more frequent. One reason for these are non-degradable deposits of degradation products. These are generated in normal cell-processes, but their accumulation during the human lifespan leads to dysfunction of cell-activity. This is believed to be the case in age related macula-degeneration, where the deposits form granules of the size of about 0.5µm to 3µm. Structured Illumination Microscopy is used to study auto-fluorescent proteins in this deposits, allowing us to separate and image them. In contrast to electron-microscopy, fluorescence microscopy has less stringent constraints to sample preparation and is generally less invasive. Additionally, it offers the possibility to separate different kinds of deposits by using multicolor excitation. This allows us to search for differences in the composition of the deposits. We analysed several histological samples by donors of different age. We present quantitative data to the relation between donor age and granula quantity. All work on human tissue was done according to the Declaration of Helsinki.