Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Posters: Imaging
BP 12.8: Poster
Dienstag, 1. April 2014, 09:30–12:30, P1
Apertureless Scanning Near-Field Optical Microscopy of Tobacco Mosaic Viruses and Intermediate Filament Desmin — •Niklas Biere1, Alexander Harder1, Mareike Dieding1, Volker Walhorn1, Sven Degenhard2, Andreas Brodehl3, Christina Wege2, Hendrik Milting3, and Dario Anselmetti1 — 1Experimental Biophysics and Applied Nanoscience, Bielefeld University — 2Dpt. of Molecular Biology and Plant Virology, Institute of Biology, University of Stuttgart — 3Heart and Diabetes Center NRW, Bad Oeynhausen
Aperture-less scanning near-field optical microscopy (aSNOM) makes use of both, high resolution topographic and fluorescence imaging. Atomic force microscopy (AFM) allows for a topographic resolution down to the atomic scale. Furthermore it can be operated in ambient conditions, liquids or in vacuum which makes it ideal for a large variety of different samples. The evanescent illumination of the AFM cantilever tip induces enhanced electromagnetic fields that are strongly confined to the tip apex. These can serve to excite and image single dye molecules. Here we report on our custom built aSNOM setup using commercially available monolithic silicon cantilevers. We investigated sparsely labelled tobacco mosaic viruses and the intermediate filament protein desmin. Both of which are complex structures composed of mixed building blocks. The simultaneous recording of topography and fluorescence data and their inherent alignment allows for the exact localization of distinct fluorescently labelled building blocks within the superordinate macromolecular structures.