Dresden 2014 – scientific programme
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BP: Fachverband Biologische Physik
BP 14: Posters: Protein Structure and dynamics
BP 14.17: Poster
Tuesday, April 1, 2014, 09:30–12:30, P1
Competing paradigms of protein dimerization tested by solution SAXS — •Stefano Da Vela1, Fajun Zhang1, Vasyl Haramus2, and Frank Schreiber1 — 1Institut für Angewandte Physik - Universität Tübingen, Tübingen, Germany — 2Helmholtz-Zentrum Geesthacht: Zentrum für Material- und Küstenforschung GmbH, Geesthacht, Germany
Small Angle X-Ray Scattering (SAXS) is a versatile and popular tool for the characterization of proteins in solution. In this contribution we use the capabilities of SAXS, Size Exclusion Chromatography and complementary Dynamic and Static Light Scattering techniques to investigate the association and interactions of Ovalbumin, Bovine Serum Albumin and Immunoglobulin G. These proteins are chosen as different paradigms for protein dimerization. We explore the architecture, the effective dimensions and hydration state of monomers and dimers in relation to the solution parameters. Furthermore, we assess the influence of the dimerization on the quality of the information which can be obtained from SAXS data and the effect of the resulting shape polidispersity on the overall protein-protein interaction parameters. A deeper insight into the dimerization process can serve as basis for further studies on its influence on protein phase behaviour and on protein dynamics in concentrated solutions.