Dresden 2014 – scientific programme
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BP: Fachverband Biologische Physik
BP 14: Posters: Protein Structure and dynamics
BP 14.6: Poster
Tuesday, April 1, 2014, 09:30–12:30, P1
Local averaging of single particle cryo-electrom microscopy data — •Amudha Kumari Duraisamy1 and Gunnar F. Schroeder1,2 — 1Institute of Complex Systems, Forschungszentrum Juelich, 52425 Juelich, Germany — 2Physics Department, Henrich-Heine-Universitaet, Duesseldorf, Germany
Single particle cryo-EM is a powerful technique to study the structure of biomolecular assemblies that are often large, flexible and conformational heterogeneous. Most of the density maps obtained from cryo-EM experiments are limited in resolution by this conformational heterogeneity. Improving the resolution of the density maps, thus, requires to account for the structural heterogeneity. In principle, the resolution can be reached to the atomic level, if the images of the structures are aligned accurately [1]. In the biological macromolecules, the conformational motions leads to global structural changes, however there are often rather rigid domains. Those rigid domains could be used to align and average the density to reach higher resolution. This is analogous to NCS averaging used to improve the phase information in the field of X-Ray crystallography [2]. An algorithm is presented to average rigid domains and to improve the resolution of cryo-EM density maps.
References: [1] Henderson. R, Q. Rev. Biophys. 28 171-193 (1995). [2] Kleywegt. G and Read. R, Structure 5 1557-1569 (1997).