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Verhandlungen
Verhandlungen
DPG

Dresden 2014 – scientific programme

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BP: Fachverband Biologische Physik

BP 2: DNA/RNA and related enzymes

BP 2.6: Talk

Monday, March 31, 2014, 10:45–11:00, ZEU 250

In situ release of DNA from artificial gene carriers — •Natalja Strelnikova1, Adriana Toma1, Rolf Dootz2, and Thomas Pfohl11University of Basel, Department of Chemistry, Switzerland — 2Max Planck Institute for Dynamics and Self Organization, Göttingen, Germany

In chromosomes DNA exists in a highly organized state, wrapped around histones, forming a composite material called chromatin. Decondensation of DNA plays an essential role in gene expression. DNA must be unpacked for transcription, arousing interest in the understanding of underlying interaction mechanisms of DNA decompaction. Our goal is to discover the real-time dynamics of the release of DNA from artificial gene carriers (dendrimer PAMAM 6/DNA). We employ a newly developed soft lithography-based microfluidics reaction device in combination with a lab source X-ray instrument. Using SAXS and SAXD (small angle X-ray scattering and diffraction), we can study the nanostructural evolution of the involved processes. We observe the dynamics of DNA release from the gene carrier using heparin as an anionic competitor. Here, negatively charged heparin competes with phosphate groups of DNA to interact with positively charged amines of PAMAM 6. The impact of the heparin and the salt concentrations as well as the pH on the disintegration of DNA/PAMAM 6 complexes can be analyzed in a temporal manner.

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