Dresden 2014 – scientific programme
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BP: Fachverband Biologische Physik
BP 30: Biomaterials and Biopolymers I (joint CPP/BP)
BP 30.2: Talk
Wednesday, April 2, 2014, 15:30–15:45, ZEU 222
Surface-Nanostructure Induced Structural and Dynamical Properties of Adsorbing Protein Layers — •Thomas F. Keller1, Robert Schulze2, Jörg Bossert2, Mark Kastantin3, Daniel K. Schwartz3, and Klaus D. Jandt3 — 1Deutsches Elektronen Synchrotron (DESY), Hamburg, Germany — 2Friedrich Schiller University Jena, Germany — 3University of Colorado Boulder, USA
Designing implant surface properties on the nanoscale may be one method for tuning the structure and dynamics of adsorbing protein layers. For a set of materials with relevance in the biomedical field, such as ultra high molecular weight polyethylene (UHMWPE), titanium dioxide (TiO2) and polystyrene-b-poly(ethylene oxide) (PS-b-PEO) block copolymers, we show how advanced materials processing permits the creation of surface nanostructures suitable for guiding adsorbing proteins into lateral arrangements that may also affect their dynamic behavior, as determined from mapping using accumulated probe trajectories (MAPT). By atomic force microscopy (AFM), we observed that 1) the surface nanostructure of native UHMWPE may establish a densely packed, ordered arrangement of fibrinogen, which is one key protein in the implant-induced blood coagulation cascade, 2) adjacent crystalline facets on a nanostructured TiO2 surface create local adsorption sites that guide fibrinogen into different conformational arrangements, and 3) nanoscale phase domains on block copolymer surfaces may serve as nucleation sites for fibrinogen networks. Ref.: ACS Nano 2011, 5, 3120; Adv. Funct. Mater. 2012, 22, 2617; Acta Biomater. 2013, 9, 5810; Macromolecules 2012, 45, 4740.