Dresden 2014 – scientific programme

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BP: Fachverband Biologische Physik

BP 30: Biomaterials and Biopolymers I (joint CPP/BP)

BP 30.8: Talk

Wednesday, April 2, 2014, 17:15–17:30, ZEU 222

Microtopographic substrates for controlling cell adhesion at the nanoscale — •Laith Kadem, Julia Purtov, Constanze Lamprecht, and Christine Selhuber-Unkel — Biocompatible Nanomaterials, Institute for Materials Science, University of Kiel

Diblock-copolymer micelle nanolithography has in recent years proven to be a valuable tool for controlling the adhesion of cells at the nanoscale by offering a control over spacing variation in binding sites of single-cell adhesion receptors. Here we present a novel method to additionally control binding sites spacing on regular micropatterns. We use a micro-structured topography on Si substrates that can be easily produced with photolithography followed by wet etching. Performing a diblock-copolymer micelle nanolithography procedure on such substrates introduces nanoparticle arrays of different densities and spacings in the pattern provided by the microtopography in a single-step. With this technique, we can achieve spacing variations in the micropattern of up to 25 nm. The microstructured domains patterned with nanoparticle arrays were biofunctionlized with RGD ligands in order to make them attractive for integrin binding in order to further study the effect of ligand spacing on cell adhesion. Thus, our micro-patterned nanostructured surfaces now provide a versatile platform for studying cellular adhesion processes that are influenced by micro-nanostructured ligand spacing and density.