Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 33: Active cell and tissue mechanics (focus session) I
BP 33.5: Vortrag
Donnerstag, 3. April 2014, 10:45–11:00, HÜL 386
Tissue packing dynamics during morphogenesis of the early Drosophila m. embryo, in toto. — •Stefan Günther1, Sebastian Streichan2, Uros Krzic3, Marvin Albert1, Timothy Saunders4, and Lars Hufnagel1 — 1European Molecular Biology Laboratory, Heidelberg, Germany — 2Kavli Institute For Theoretical Physics, USA — 3Carl Zeiss Microscopy, TASC, Munich, Germany — 4Mechanobiology Institute and Department of Biological Sciences, Singapore
The organization of tissues is challenged by the propagation dynamics of the cells which constitute the tissue. How the dynamic motion shape tissues in vivo during early development is poorly understood due to a lack of quantifiable data. We use a selective plane illumination microscope (MuVi-SPIM) and automated image analysis to quantify the dynamics of the nuclei packing in 3D. High temporal and spatial resolution allows us to analyze the relationship between the packing of nuclei in the entire embryo and the orientation of the division axis of nuclei. Lineages of all nuclei through several rounds of division are used to explore the role of a nucleus' spatio-temporal history for its local packing. We further use computational models in order to propose the necessary interactions that can lead to the orderliness and the dynamics of the observed nuclei packing and test the predictions using laser ablations to locally perturb the developing embryo.