Dresden 2014 – scientific programme
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BP: Fachverband Biologische Physik
BP 34: Imaging
BP 34.8: Talk
Thursday, April 3, 2014, 11:30–11:45, ZEU 250
Detection of vancomycin resistances in enterococci using a combined dielectrophoresis-Raman setup and a three-level chemometric model — •Ulrich Schröder1, 2, Cora Assmann2, Claudia Beleites1, Uwe Glaser1, 2, Uwe Hübner1, Wolfgang Pfister4, Wolfgang Fritzsche1, Jürgen Popp1, 2, 3, and Ute Neugebauer1, 2 — 1Institute of Photonic Technology, Jena, Germany — 2Center for Sepsis Control and Care, Jena University Hospital, Germany — 3Institute of Physical Chemistry and Abbe Center of Photonics, University Jena, Germany — 4Institute of Medical Microbiology, Jena University Hospital, Germany
The rising resistances of pathogens towards antibiotics represent a significant problem in human health-care. In this context, enterococci have become one of the most challenging nosocomial problems. It is of utmost interest to detect these resistances early in time to initiate appropriate tailored antibiotic therapies. We present a combined DEP-Raman setup which traps bacteria directly from dilute suspension within micro sized regions so as to perform highly specific Raman spectroscopic analysis. The setup is used to analyze the response of sensitive and resistant enterococci with respect to the antibiotic vancomycin. In combination with a three-level chemometric model based on PLS and LDA we are able to detect the induced drug resistance within less than three hours. Compared to standard microbiological methods which take 24 hours and more our method holds the potential to reduce diagnosis time by orders of magnitude. Acknowledgement: Financial support of BMBF (FKZ 01EO1002) is highly acknowledged.