Dresden 2014 – scientific programme

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BP: Fachverband Biologische Physik

BP 39: The Collapsed State of Polymers: From Physical Concepts to Applications and Biological Systems (accompanying session, joint CPP/BP/DY)

BP 39.6: Talk

Thursday, April 3, 2014, 16:45–17:00, ZEU 250

Effects of nucleosome positioning on condenstation of short and long chromatin fibers — Robert Schöpflin¹, Oliver Müller¹, Christin Weinberg¹, Vladimir B. Teif², Karsten Rippe², and •Gero Wedemann¹ — ¹CC Bioinformatics, University of Applied Sciences Stralsund, Stralsund, Germany — ²Deutsches Krebsforschungszentrum & BioQuant, Heidelberg, Germany

In eukaryotes DNA is associated with proteins in a complex structure termed chromatin. The basic packaging unit of chromatin is the nucleosome in which DNA is wrapped around a histone octamer. Experiments indicate that chromatin has different packaging conditions connected to distinct activation states. Experimental evidence showed that packaging and activation states are closely linked to positions of nucleosomes on the DNA which are actively regulated. To improve the understanding of the interplay between nucleosome positions and chromatin structure we applied computer simulations of a coarse-grained chromatin model including fundamental physical properties such as elasticity, electrostatics and nucleosome interactions using a feedback-optimized replica exchange protocol. We calculated the effect of nucleosome positioning on the structure of polynucleosomes of different length scales, up to the size of a gene locus. We compared chromatin models based on synthetic positions with models based on experimentally derived nucleosome positions from cells at different stages of cell differentiation. Simulation results revealed a significant influence of nucleosome positions on the three dimensional structure of chromatin.