Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 4: Membranes and Vesicles I
BP 4.3: Vortrag
Montag, 31. März 2014, 15:45–16:00, HÜL 386
Artificial DNA Membrane Nanopores — •Kerstin Göpfrich1, Jonathan Burns2, Vivek Thacker1, Thomas Zettl1, Silvia Hernandez-Ainsa1, Eugen Stulz3, Stefan Howorka2, and Ulrich Keyser1 — 1Cavendish Laboratory, University of Cambridge, UK — 2Department of Chemistry, University College London, UK — 3Department of Chemistry, University of Southampton, UK
Membrane nanopores are essential components of biological and artificial cells. Our group has shown that we can create artificial nanopores using DNA origami self-assembly (N.A.W. Bell, Nanoletters, 2012; S.M. Hernandez-Ainsa, ACS nano, 2013) and anchor them in lipid membranes (J. Burns, K. Göpfrich, Angewandte Chemie, 2013).
Insertion of negatively charged DNA pores into a hydrophobic membrane is achieved by attaching functional hydrophobic groups in strategic positions on the DNA nanopores. Pore formation in lipid vesicles is studied for different nanopore designs and hydrophobic modifications via fluorescent imaging (V.V. Thacker, Applied physics letters, 2012). Single-channel current recordings of our artificial DNA nanopores are performed using a high-throughput lipid nanobilayer system that has recently been introduced by our group (K. Göpfrich, Langmuir, 2013; J.L. Gornall, Nano letters, 2011). Pore architecture and functionality of our DNA nanopores can be easily adapted, opening the pathway to design novel membrane channels.