Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 41: Biomaterials and Biopolymers II (joint CPP/BP)
BP 41.9: Vortrag
Donnerstag, 3. April 2014, 17:30–17:45, ZEU 222
Diffusion regulation in the basal lamina — •Fabienna Arends1,2 and Oliver Lieleg1,2 — 1Zentralinstitut für Medizintechnik, Technische Universität München, Boltzmannstr.11, 85748 Garching — 2Fakultät für Maschinenwesen,Technische Universität München, Boltzmannstr.15, 85748 Garching
The permeability of the basal lamina, a biological hydrogel found at the basolateral side of the epithelium, is an important property for the design of both new drug delivery systems and biomimetic hydrogels. Moreover, it is highly desirable to understand the diffusion of colloidal particles and macromolecules such as drug delivery vehicles, nutrients, growth factors, and proteins across the basal lamina. The mobility of those objects in this highly complex gel is regulated by a broad range of factors including geometric constraints and different types of physical interactions between the particles/molecules and the hydrogel constituents.
Here, we quantify the diffusion of colloids and molecules within an extracellular matrix gel (ECM) purified from the Engelbreth-Holm-Swarm sarcoma, which is a model system for the basal lamina. For this quantification we use single particle tracking techniques and measure the formation of a concentration gradient of solutes across the ECM. From our data we aim at deciphering the underlying mechanisms responsible for the permeability properties of the hydrogel.