Dresden 2014 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 42: Biotechnology and bioengineering

BP 42.8: Vortrag

Freitag, 4. April 2014, 11:30–11:45, HÜL 386

Label free biomolecular interaction studies with imaging ellipsometry * an Overview. — •Peter H. Thiesen — Accurion GmbH, Göttingen, Germany

Interactions between biomolecules play a central roles in every life process. Surface plasmon resonance (SPR) in Kretschmann configuration is state of the art in bio molecular interaction analysis, but a number of papers in literature show that the high lateral resolution, the capability of mapping and ellipsometric contrast micrographs performed by Imaging SPR in the ellipsometric mode or imaging surface plasmon resonance enhanced ellipsometry (i-SPREE) is promising for the development of new options in detection and screening of biomolecular interaction. Valiokas et al. [1] characterized differential protein assemblies on micro patterned surfaces with nitriletriacetic acid based surface functionalization. Klenkar et al. [2] used the technique to follow the addressable adsorption of lipid vesicles and subsequent protein interaction studies and to detect Narcotics trace detection [3]. Schuy et al. report a mimetic approach to the active drug target for fusion inhibitors of HIV (human immunodeficiency virus) and SIV (simian immunodeficiency virus) [4]. Beside reviewing, new applications in the field of protein adsorption, protein/protein and single strain DNA interaction, the capability of imaging ellipsometry in QC of arrays and also basic surface coating will be addressed. [1] Valiokas et al., ChemBioChem 7, 1325 (2006) [2] Klenkar et al., Biointerphases 3, 29(2008) [3] Klenkar et al., Anal Bioanal Chem 391, 1679(2008) [4] Schuy et al., Journal of Structural Biology 168, 125 (2009)