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BP: Fachverband Biologische Physik
BP 6: Posters: Membranes and vesicles
BP 6.2: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Lipid bilayers on microporous substrates — •Theresa Kaufeld1, Claudia Steinem2, and Christoph F. Schmidt1 — 1Drittes Physikalisches Institut - Biophysik, Georg-August Univesität Göttingen — 2Institut für organische und biomolekulare Chemie, Georg-August Universität Göttingen
We have designed microporous substrates with individually addressable arrays of micrometer-sized apertures for a combination of electrical experiments and fluorescence microscopy, which are also suitable for other techniques such as mechanical manipulation of lipid bilayers. An integrated electrode facilitates access for microscope objectives. We characterized the substrates in terms of surface roughness and pore geometry by SEM and AFM. We then used impedance spectroscopy to characterize the substrate electrically with and without the integrated electrode and measured a resistance in the kilo-Ohm range, which is clearly distinguishable from the Giga-Ohm seal we found with pore-spanning lipid bilayers. Solvent-free lipid bilayers were formed by GUV spreading and imaged with fluorescence microscopy. Electric experiments using alamethicin as a test ion channel showed single-channel resolution.