Dresden 2014 – scientific programme

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BP: Fachverband Biologische Physik

BP 7: Posters: Cell adhesion, mechanics and migration

BP 7.14: Poster

Monday, March 31, 2014, 17:30–19:30, P3

Temperature induced sudden loss of cell nuclei integrity — •Enrico Warmt, Tobias Kießling, Roland Stange, Anatol Fritsch, Mareike Zink, and Josef Käs — Universität Leipzig, Experimentelle Physik I, Germany

The DNA double helix, is one of the most stable proteins in a cell. However, despite the high temperature stability of DNA itself, we have found a sudden loss of cell nuclei integrity at relative moderate temperatures ranging from 45 to 55 degree Celsius. Suspended cells hold in an optical double beam trap were heated under controlled conditions and nuclear shape was monitored. At specific critical temperatures an irreversible sudden shape transition of the nuclei was observed. These temperature induced transitions differ in character of shape change for different cell lines. The high connectivity of the nuclei to the cytosol becomes visible when the initial shape transition of the nucleus propagates toward the plasma membrane.