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BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.22: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Resolution Limits and Regularization in Traction Force Microscopy — •Jérôme R. D. Soiné1,2, Christoph A. Brand1,2, and Ulrich S. Schwarz1,2 — 1Institute for Theoretical Physics, Heidelberg University, Heidelberg, Germany — 2Bioquant, Heidelberg University, Heidelberg, Germany
Regularization is a standard technique to achieve unique solutions for inverse problems that are ill-posed. One such problem is reconstructing cellular traction fields of adherent cells on planar elastic substrates, a method known as traction force microscopy (TFM). Regularization is based on the idea of suppressing noise effects. However, in TFM the choice of the regularization term and of the regularization strength may affect reconstruction results such as the spatial distribution of traction hot spots, traction magnitudes and feature sizes. We have conducted a systematic investigation of the effects of using different regularization schemes in TFM. In contrast to unconstrained TFM, we constrained cellular force transmission to specific locations identified from fluorescence microscopy data for proteins localizing to focal adhesions. We then correlated the properties of these focal adhesions with the traction forces reconstructed by different methods. This enabled us to give a systematic overview on how regularization influences the reconstruction of cellular traction fields and to provide theoretical tools to adjust regularization for various experimental environments. We also compare our results to model-based traction force microscopy (MB-TFM), a method in which biophysical models are used to further suppress the effect of noise and to extract more information.