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BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.37: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Modeling cytoskeletal polarization during confinement-induced persistent amoeboid motion — •Oliver Nagel1, Can Guven2, Matthias Theves1, Meghan Driscoll2, Wolfgang Losert2, and Carsten Beta1 — 1Institute of Physics and Astronomy, University of Potsdam, Germany — 2Department of Physics, University of Maryland, MD, USA
We studied the quasi one-dimensional motion of Dictyostelium discoideum amoebae inside narrow microfluidic channels with a cross section of 10 x 20 micrometer. Most of the cells performed a quasi one-dimensional random walk under these conditions. However a subpopulation of cells showed a completely different type of motion. They persistently moved in one direction for a long time without reversing or stopping. We performed laser scanning confocal imaging of a transfected Dictyostelium cell line that expressed myosin II-GFP together with LimE-mRFP, a marker for filamentous actin. Our experiments showed, that the polarized structure of the cell cortex was different from those of polarized cells in the absence of confinement. To systematically analyze the dynamics of local protrusions and retractions of the membrane, we used a custom made software tool for cell shape analysis. Taking into account the observed distributions of actin and myosin II in the cell cortex, we developed a model, based on the biased excitable network model by Iglesias and Devreotes [1] to describe this behavior. Ref.: 1. P. Iglesias and P. Devreotes, Current Opinion in Cell Biology, 24 (2012), 245*253 <doi:10.1016/j.ceb.2011.11.009>.