Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.41: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Alteration of rolling adhesion in aged monocytes — •Samira Khalaji1, Kay-E Gottschalk1, Lisa Zondler2, Veselin Grozdanov2, and Karin Danzer2 — 1Institut für experimentelle Physik, 89081 Ulm, Germany — 2Institut für Neurologie , 89081 Ulm, Germany
Cells alter adhesion to the cells or extracellular matrix during tissue remodeling, morphogenesis, and other responses to the environmental signals. Adhesion of blood monocytes was measured by setting up flow experiments using unidirectional laminar flow and low shear stress (0.59-1 dyn/cm2). Cells were isolated from young adult (20-40 years) and older adult (+40 years) donors and cultured 24 hours with/without Lipopolysaccharide (LPS) before running the flow experiments. Number of rolling and firmly adhesive cells to the substrate during the time point of 7-10 minutes was quantified using live cell imaging, plotted and compared in respect to the age.