Dresden 2014 – scientific programme
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BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.6: Poster
Monday, March 31, 2014, 17:30–19:30, P3
T-cell response to ligand presentation in the form of nano-dot arrays — •Fuwei Pi1, Pierre Dillard1,2, Anne Charrier1, Laurent Limozin2, and Kheya Sengupta1 — 1Aix-Marseille Université, CNRS, CINaM UMR 7325, Marseille, 13288, France — 2Laboratoire Adhésion & Inflammation, Aix-Marseille Université / Inserm U1067 / CNRS UMR7333, Marseille, 13288, France
We developed a simple cost-effective benchtop protocol to functionalize glass or polydimethylsiloxane elastomers with an array of nanometric protein dots for cell adhesion studies [1]. The diameter of the dot was varied down to about 80 nm. The adhesion of T-lymphocytes (Jurkat) on substrates patterned with the ligand anti-CD3 was studied using a variety of imaging techniques. The adhesion area was quantified using reflection interference contrast microscopy (RICM). Total internal reflection fluorescence (TIRF) microscopy was used to explore the possible colocalization of T-cell receptor microclusters and the activating anti-CD3 dots. The impact on the structure of the actin cytoskeleton was imaged with TIRF and confocal microscopy. We report modulation of the cell surface topography, and actin organization.
[1] F. Pi, P. Dillard, L. Limozin, A. Charrier, K. Sengupta; NanoLett. 13 7 3372-3378 (2013).