Dresden 2014 – scientific programme
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BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.7: Poster
Monday, March 31, 2014, 17:30–19:30, P3
Regulation of Hematopoietic Stem Cell Adhesion by Nanometric Presentation of Niche Ligand Candidates — •Alexandra Burk1,2, Cornelia Monzel2, Hiroshi Yoshikawa3, Anthony Ho4, and Motomu Tanaka1,2,5 — 1Institute for Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany — 2Physical Chemistry of Biosystems, Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany — 3Department of Chemistry, Saitama University, Saitama, Japan — 4Clinic for Internal Medicine V, Heidelberg University, Heidelberg, Germany — 5Institute for Integrated Cell-Material Sciences (WPI iCeMS), Kyoto University, Kyoto, Japan
A major challenge in understanding the mobilization/immobilization mechanisms of hematopoietic stem cells (HSC) is to characterize the complex interplays of HSC-niche interactions in bone marrow such as the receptor/ligand pairs N-cad/N-cad and CXCR4/SDF1α. To quantify these interactions, we designed well defined niche models consisting of planar lipid membranes on solid substrates displaying recombinant N-cadherin and human SDF-1α at defined intermolecular distances (5 nm to 100 nm). For contact- and label-free imaging of HSC, micro-interferometry (RICM) and phase contrast imaging was applied. To evaluate the strength of cell adhesion, we developed a new technique utilizing pressure waves induced by a picosecond laser pulse for non-invasive HSC detachment. Moreover, time-lapse analysis of cellular motion resulted in characteristic mean square displacements and modes of motion depending on the underlying substrate.