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BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.9: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Correlation analysis of the role of TRPC6 channels in the regulation of CXCR2-mediated chemotaxis of murine neutrophils — •Peter Dieterich1, Otto Lindemann2, and Albrecht Schwab2 — 1Institut für Physiologie, TU Dresden — 2Institut für Physiologie II, Westfälische Wilhelms-Universität Münster
Cellular motility and the ability of cells to sense and react to changes of their environment are of fundamental importance for efficient immune response. Chemoattractants trigger receptor initiated signaling cascades including the activation of plasma membrane Ca2+ channels of the transient receptor potential channel family (TRPC). Here we disentangle the influence of TRPC6 channels on cell migration paths of murine neutrophils during chemotaxis caused by spatially increasing concentrations of keratinocyte-derived cytokine KC. Wildtype neutrophils show directed motion and diffusion. Blocking of the KC-receptor CXCR2 with a specific inhibitor reduces directed motion to ∼ 25% compared to wildtype cells and simplifies velocity autocorrelations to an exponential decay. Knock-out of TRPC6 channels results in reduced directed motion to ∼ 20%. However, stronger temporal autocorrelations of the migration process are conserved. In addition, data are assessed with a generalized Langevin equation, allowing to separate the migration pattern into motor and navigation system and to quantify intrinsic correlation more precisely.