Dresden 2014 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 33: Cytoskeleton (original: BP, joined by CPP)

CPP 33.10: Talk

Wednesday, April 2, 2014, 12:15–12:30, HÜL 386

Fluorescent beads disintegrate actin networks — •Tom Golde, Carsten Schuldt, Jörg Schnauß, Dan Strehle, Martin Glaser, and Josef Käs — Institut für Experimentalphysik 1, Universität Leipzig, Leipzig, Deutschland

We studied the influence of fluorescent polystyrene beads on both entangled and cross-linked actin networks. Thermal bead fluctuations were observed via video particle tracking and analyzed with one-point microrheology. Illumination of fluorescent beads with their appropriate excitation wavelength leads to a drastic softening of actin gels. Other wavelengths and bright field microscopy do not increase thermal bead fluctuations. This effect cannot be significantly reduced by adding common oxygen scavengers. We conclude that the usage of fluorescent beads impairs results when studying the microrheology of actin networks [1].

[1] Golde et al., Physical Review E 88, 044601 (2013)