Dresden 2014 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 37: Poster Session 2
CPP 37.24: Poster
Mittwoch, 2. April 2014, 15:00–19:00, P3
Micro-optical lens array for ultrahigh-throughput fluorescence detection in droplet-based microfluidics — •Jiseok Lim1,2, Philipp Gruner1, Jeremy Vrignon1, Christos S. Karamistos2, Manfred Konrad2, and Jean-Christophe Baret1 — 1Max Planck Institute for Dynamics and Self-organization, Am Fassberg 17, D-37077 Goettingen, Germany — 2Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Goettingen, Germany
Droplet-based microfluidic systems are promising platforms for high-throughput screening applications. Elementary methods for droplet production, incubation, fusion, and sorting, have been developed over the past years to provide parallelized droplet manipulation required for these applications. Detection systems have so far been poorly parallelized, setting up a bottle neck for the increase of throughput. We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals. We show that the integration of microlenses and mirror surfaces in these devices results in an 8-fold increase of the fluorescence signal and in improved spatial resolution [1]. Finally, we demonstrate that the developed system can be used to detect beta-galactosidase activity of single E.coli cells in 100pL droplets. We reached a throughput more than 100 000 droplets per second, an analytical throughput larger than those obtained using flow cytometry [2]. References: [1] J. Lim et al., Lab Chip, 13, 1472-1475 (2013) [2] J. Lim et al., Appl. Phys. Lett., 103, 203704 (2013)