Dresden 2014 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 37: Poster Session 2

CPP 37.41: Poster

Mittwoch, 2. April 2014, 15:00–19:00, P3

Salt Induced Reduction of Lysozyme Adsorption at Behenic Acid Membranes — •Holger Göhring, Michael Paulus, and Metin Tolan — Fakultät Physik/DELTA,Technische Universität Dortmund, D-44221 Dortmund

Lipid membranes serve in biological cells as boundaries between intracellular and extracellular regions. Many biological processes taking place at membranes are associated with the adsorption of proteins. We present a study on the adsorption behaviour of the protein lysozyme at behenic acid films. In the case of electrostatic interaction between proteins and membranes, the screening effect of salt ions can be used to control the adsorption. Therefore we focus the investigation on the effect of NaCl on the adsorption of lysozyme. By varying the salt concentrations the electrostatic interaction between the positively charged proteins and the negatively charged head groups of the behenic acid is altered resulting in a decreased lysozyme adsorption with rising NaCl concentration. The adsorption process is investigated by x-ray reflectivity measurements, which allow the determination of the electron density profiles of thin layers at the liquid -- gas interface.