Dresden 2014 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 51: Charged Soft Matter II
CPP 51.2: Vortrag
Donnerstag, 3. April 2014, 15:15–15:30, ZEU 118
LCST phase behavior in protein solutions — •Michal Braun1, Marcell Wolf1, Felix Roosen-Runge1, Fajun Zhang1, Roland Roth2, and Frank Schreiber1 — 1Institut für Angewandte Physik - Universität Tübingen, Auf der Morgenstelle 10, 72076 Tübingen — 2Institute for Theoretical Physics - Universität Tübingen, Auf der Morgenstelle 14, 72076 Tübingen
The phase behavior of BSA and HSA (bovine and human serum albumin) solutions containing yttrium (Y3+) is investigated. For both proteins, a phase diagram in dependence of protein and salt concentration (cp and cs) at room temperature has been established ([1], [2]). In a certain regime, the solutions are turbid which is either due to aggregation or liquid-liquid phase separation (LLPS). The 3-D shape of the LLPS binodal for BSA depending on cp, cs and temperature T has been measured. A lower critical solution temperature (LCST) phase behavior has been observed. Dynamic and static light scattering measurements are currently performed in order to monitor the diffusion coefficient as well as the static correlation length while the system approaches the binodal. The molecular mechanisms behind this behavior will be investigated in the future, considering the competing effects of ion bridging and hydration.
[1] F. Zhang et al., Phys. Rev. Lett. 101 (2008) 148101.
[2] F. Zhang et al., Soft Matter 8 (2012) 1313.