Dresden 2014 – scientific programme
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DF: Fachverband Dielektrische Festkörper
DF 10: Dielectric surfaces and interfaces
DF 10.1: Talk
Wednesday, April 2, 2014, 09:30–09:50, GER 37
Aligned deposition of λ-DNA on LiNbO3 substrates by means of Ferroelectric Lithography — •Saminathan Ramakrishnan, Alexander Haußmann, and Lukas Eng — Institut für Angewandte Photophysik, TU Dresden, Dresden, Germany.
We report on the controlled deposition of λ-DNA to domain walls (DWs) of 5 mol.% Mg doped congruent LiNbO3. Firstly, DWs were photochemically decorated with Au nanoparticles serving as pinning points to graft the DNA molecules [1]. Subsequently,these pin points were functionalized with 1,6-hexanedithiol, thus providing free reactive thiol groups at the Au surface. Incubation of fluorescently (YOYO-1) labeled λ-DNA followed by an intensive washing with a buffer solution that finally resulted in single DNA molecules that bridge the gaps between the aforementioned pin points. The analysis of these multi-component nanostructures and the underlying domain pattern was carried out by means of optical fluorescence microscopy, dynamic mode scanning force microscopy, and piezoresponse microscopy.
[1] A. Haußmann et al., Nano Lett. 9, 763 (2009)