Dresden 2014 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 31: Molecular Films: Properties and Preparation
O 31.10: Vortrag
Dienstag, 1. April 2014, 12:45–13:00, WIL A317
Biomimetic solid-supported polymer bilayer incorporated with natural membrane proteins — •xiaoyan zhang1, wangyang fu2, cornelia g. palivan1, and wolfgang meier1 — 1Department of Chemistry, University of Basel, Klingelbergstrasse 80, CH-4056 Basel, Switzerland — 2Department of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland
Lipid cell membrane is the most important interface in biological systems, which is able to selectively control the transport by their specific proteins. Reconstitution of membrane proteins in artificial membranes creates a platform for exploring their potential for pharmacological or biotechnological applications. Previously, we demonstrated amphiphilic block copolymer is a relatively stable substitute for a lipid and is able to be used as promising building blocks for artificial membranes with long-term stability and tailorable structural parameters. However, the insertion of membrane proteins has not previously been realized in a planar large-area, stable, and solid-supported artificial membrane. Herein we present our recent work on the first, preliminary model of a channel protein that is incorporated in block copolymer, tethered, solid-supported bilayer membrane (TSSBM). Unprecedented ionic transport characteristics that differ from previous results on protein insertion into planar, free-standing membranes, are identified. For the unique variation in conductance in a TSSBM, a model describing channel protein mediated ion transport was introduced. Our findings mark a change in understanding protein insertion and ion flow within natural channel proteins when inserted in an artificial TSSBM.