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BP: Fachverband Biologische Physik

BP 1: Imaging

BP 1.8: Vortrag

Montag, 16. März 2015, 11:45–12:00, H 1028

PEEM and SEM of Magnetospirillum magnetotacticum’s Magnetosome Chains — •Christoph Keutner¹, Alex von Bohlen², Ulf Berges¹, Philipp Espeter¹, Claus M. Schneider³, and Carsten Westphal¹ — ¹DELTA/Experimentelle Physik I, TU Dortmund, Maria-Goeppert-Mayer-Straße 2, 44221 Dortmund, Germany — ²ISAS Dortmund, Bunsen-Kirchhoff-Straße 11, 44139 Dortmund, Germany — ³PGI-6, FZ Jülich, 52425 Jülich, Germany

Magnetotactic bacteria are of great interdisciplinary interest, since a vast field of applications from magnetic recording media to medical nanorobots is conceivable. A key feature for a further understanding is the detailed knowledge about the magnetosome chain within the bacteria.

Here, we present two preparation procedures of Magnetospirillum magnetotacticum suitable for UHV experiments. These allow us to preform photoemission electron microscopy (PEEM) on magnetotactic bacteria, for the first time. We show that PEEM combined with x-ray absorption spectra (XAS) can access the magnetic particles (magentosomes) within intact magnetotactic bacteria.

By combining scanning electron microscopy (SEM) with energy dispersive x-ray spectrometry (EDX), magnetosome chains of intact bacteria become directly visible through the cell envelope. Even single magnetosomes as individual parts of the chains can be imaged.