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Berlin 2015 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Colloids and Complex Liquids II (joint CPP/DY/BP)

BP 10.12: Vortrag

Montag, 16. März 2015, 18:00–18:15, C 130

A systematic study of the influence of trivalent metal ions on phase behaviour in protein solutions — •Olga Matsarskaia1, Michal Braun1, Andrea Sauter1, Marcell Wolf1, Roland Roth2, Fajun Zhang1, and Frank Schreiber11Institut für Angewandte Physik, Universität Tübingen, 72076 Tübingen — 2Institut für Theoretische Physik, Universität Tübingen

Thermodynamic phenomena such as reentrant condensation (RC) and liquid-liquid phase separation (LLPS) are involved in various protein-related processes, e.g. protein condensation diseases and protein crystallisation. We could show that these transitions are inducible in protein solutions using various trivalent cations [1], [2]. In this work, the influence of cation size on such phase behaviour in bovine serum albumin (BSA) was studied systematically in the presence of salts with increasing cation sizes (YbCl3, YCl3, GdCl3, CeCl3 and LaCl3). The results reveal that charge inversion, the prerequisite of RC and LLPS in these systems, is found independent of cation size. Interestingly, however, salt concentration ranges in which macroscopic LLPS is observed decrease with increasing cations: while Yb3+ leads to the largest LLPS area, Ce3+ features the smallest one. La3+, the largest cation studied, induces RC, but does not lead to LLPS at all. The findings thus indicate that the size of cations present in the environment of a protein influences the strength of protein-cation interactions and therefore plays an important role in phase transitions of the protein.

[1] Zhang et al (2008). Phys. Rev. Lett., 101(14), 148101; [2] Zhang et al (2012). Soft Matter, 8, 1313-1316.

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